Extracting DNA for genotyping

Lysis buffer

100ml 1M TrisHCl (pH 8.5)

500ml 10mM EDTA

10ml 20% SDS

11.688g NaCl

dH2O to 1litre

 

Note

Add 250ul of proteinase K (20mg/ml) to 50ml of lysis buffer directly before use.

 

Procedure

chop up tail tips
             ↓
add 500ul lysis buffer with proteinase K per eppendorf with tissue sample
             ↓
incubate overnight at 55ºC

 

Notes

  1. crude DNA extraction can be used in some genotyping PCR reactions
  2. otherwise, phenol: chloroform extract the DNA before use as per protocol