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- SYBIL at a glance
- Bone
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- Diastrophic dysplasia
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- PSACH and MED
- Systems biology
- SOPs
- Alcian Blue staining
- Bone measurements
- BrdU labelling
- Cell counting using ImageJ
- Chondrocyte extraction
- Cre genotyping protocol
- DMMB assay for sulphated proteoglycans
- Densitometry using ImageJ
- Double immunofluorescence
- Electron microscopy of cartilage - sample prep
- Extracting DNA for genotyping
- Grip strength measurement
- Histomorphometry on unon-decalcified bone samples
- Immunocytochemistry
- Immunofluorescence
- Immunohistochemistry
- Quantitative X-ray imaging on bones using Faxitron and ImageJ
- Skeletal preps
- TUNEL assay (Dead End Fluorimetric Kit, Promega)
- Toluidine Blue staining
- Toluidine Blue staining
- Von Kossa Gieson staining
- Wax embedding of cartilage tissue
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SOPs
Stardard Operating Procedures (research protocols) in bone and cartilage biology:
- Acrylate Histology (UKE)
- Alcian Blue staining (UNEW)
- Bone measurements (UNEW)
- BrdU labelling (UNEW)
- Cell counting using ImageJ (UNEW)
- Chondrocyte extraction (UNEW)
- Cre genotyping protocol (UNEW)
- Densitometry using ImageJ (UNEW)
- DMMB assay (UNEW)
- Double immunofluorescence (UNEW)
- Electron microscopy of cartilage (UNEW)
- Extracting DNA for genotyping (UNEW)
- Grip strength measurement (UNEW)
- Histomorphometry of non-decalcified bone (UNIPV)
- Immunocytochemistry (UNEW)
- Immunofluorescence (UNEW)
- Immunohistochemistry (UNEW)
- Quantitative X-ray imaging of bones using ImageJ (UNEW)
- Skeletal preps (UNEW)
- TUNEL assay (UNEW)
- Toluidine Blue staining (UKE)
- Toluidine Blue staining (UNEW)
- Wax embedding of cartilage tissue (UNEW)
- Von Kossa (UKE)
